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Distinctive dual indexes minimize for each-sample Value and sequence libraries with the very best accuracy when compared to other indexing procedures.
Index one: the i7 index column is inside the ahead orientation (match the orientation if the i7 indexes during the sample sheet).
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When demultiplexing, the final sample sheet offered into the FASTQ generation computer software should contain the i5 index in the correct orientation for that System. Having said that, most Illumina sample sheet era application handles this routinely.
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Set C Index Adapters Refer to Index two (i5) Orientation To learn more on how to enter i5 bases around the sample sheet in ahead or reverse enhance orientation.
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When applying BaseSpace Sequence Hub PrepTab, i5 sequences have to be entered into these computer software from the ahead orientation in all situations and for all instrument types.
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